Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

v

Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

ix

Benchtop Bioreactors in Mammalian Cell Culture: Overview

and Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1

Ange´lique Schmid, Emanuel Kreidl, Martin Bertschinger,

and Patrick Vetsch

Volumetric Mass Transfer Coefficient Measurement in a Stirred

Tank Reactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

17

Aysegul Inam, Ezgi Rojda Taymaz, Mehmet Emin Uslu,

Baris Binay, and Irem Deniz

Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter

Volume Bioreactors for Cell Culturing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

27

Elif Gencturk, Senol Mutlu, and Kutlu O. Ulgen

Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors. . . . . . . . . . .

39

Marites T. Woon, Puspa R. Pandey, and Inbar Friedrich Ben-Nun

High-Efficiency Differentiation of Human Pluripotent Stem Cells

to Hematopoietic Stem/Progenitor Cells in Random Positioning

Machine Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

55

Xiaohua Lei, Chiyuan Ma, Yujing Cao, Yue Xiong,

Jian V. Zhang, and Enkui Duan

Integrating Human-Induced Pluripotent Stem Cell Expansion

Capability and Cardiomyocyte Differentiation Potential in a Microcarrier

Suspension Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

67

Valerie Ho, Gerine Tong, Alan Lam, Shaul Reuveny, and Steve Oh

Chemically Defined, Xeno-Free Expansion of Human Mesenchymal

Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use

Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

83

Misha Teale, Valentin Jossen, Dieter Eibl, and Regine Eibl

Large-Scale Expansion of Umbilical Cord Mesenchymal Stem

Cells with Microcarrier Tablets in Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

113

Huanye Xu, Zhongxiao Cong, Yuanyuan Zhang, Wei Liu,

Xiaojun Yan, and Yanan Du

Optimized Method to Improve Cell Activity in 3D Scaffolds

Under a Dual Real-Time Dynamic Bioreactor System. . . . . . . . . . . . . . . . . . . . . . . . . . . .

127

Flavia Pedrini, Moema A. Hausen, and Eliana A. R. Duek

In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells

by Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

135

Ziming Chen, Peilin Chen, Rui Ruan, and Minghao Zheng

Microcarrier-Supported Culture of Chondrocytes in Continuously

Rocked Disposable Bioreactor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

145

Kamil Wierzchowski and Maciej Pilarek

vii